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zeb1 (Bethyl)

Name: zeb1
Brand: Bethyl
Rating: 93 (63 reviews)

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Bethyl zeb1
Zeb1, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/zeb1/product/Bethyl
Average 93 stars, based on 63 article reviews

zeb1 - by Bioz Stars, 2026-03

93/100 stars

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A 29 ferroptosis-related genes related to RBMS3-AS3 expression were screened in the heatmap of co-expression analysis. B , C The expression of ZEB1, PTEN, and ATM was examined by RT-qPCR and WB in A549 and PC9 cells knocking down or overexpressing RBMS3-AS3. D , E ZEB1 was downregulated in LUAD tissues from TCGA and GTEx cohorts ( D ) and the JSPH cohort ( E ). F The RNA levels of ZEB1 in 32 paired LUAD samples from JSPH were measured by RT-qPCR. G RBMS3-AS3 expression showed a positive correlation with ZEB1 RNA levels in 32 paired LUAD samples from JSPH. H , I The decreased expression of ZEB1 predicted an unfavorable patient OS in the GSE31210 and GSE3141 cohorts. J , K The intracellular lipid ROS of A549 and PC9 cells treated with RSL3 were examined by C11-BODIPY581/591 following the modulation of ZEB1 expression. L – Q The role of ZEB1 in modulating the intracellular ferroptosis level in LUAD cells was detected by measuring the GSH/GSSG ratio ( L , M ), Fe 2+ level ( N , O ), and MDA level ( P , Q ). Data were representative images or were expressed as the mean ± standard deviation. ns: no significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: NPJ Precision Oncology

Article Title: M6A-ALKBH5-dependent RBMS3-AS3 down-regulation suppresses ferroptosis to promote lung adenocarcinoma progression through HNRNPDL/ZEB1/GPX4 axis

doi: 10.1038/s41698-025-01141-y

Figure Lengend Snippet: A 29 ferroptosis-related genes related to RBMS3-AS3 expression were screened in the heatmap of co-expression analysis. B , C The expression of ZEB1, PTEN, and ATM was examined by RT-qPCR and WB in A549 and PC9 cells knocking down or overexpressing RBMS3-AS3. D , E ZEB1 was downregulated in LUAD tissues from TCGA and GTEx cohorts ( D ) and the JSPH cohort ( E ). F The RNA levels of ZEB1 in 32 paired LUAD samples from JSPH were measured by RT-qPCR. G RBMS3-AS3 expression showed a positive correlation with ZEB1 RNA levels in 32 paired LUAD samples from JSPH. H , I The decreased expression of ZEB1 predicted an unfavorable patient OS in the GSE31210 and GSE3141 cohorts. J , K The intracellular lipid ROS of A549 and PC9 cells treated with RSL3 were examined by C11-BODIPY581/591 following the modulation of ZEB1 expression. L – Q The role of ZEB1 in modulating the intracellular ferroptosis level in LUAD cells was detected by measuring the GSH/GSSG ratio ( L , M ), Fe 2+ level ( N , O ), and MDA level ( P , Q ). Data were representative images or were expressed as the mean ± standard deviation. ns: no significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The DNA fragments were subjected to immunoprecipitation with the primary antibody against ZEB1 (21544-1-AP, Proteintech, Wuhan, China) and normal rabbit IgG (30000-0-AP, Proteintech, Wuhan, China).

Techniques: Expressing, Quantitative RT-PCR, Standard Deviation

A – D The rescue CCK-8 assay ( A ), colony formation assay ( B ), EdU staining assay ( C ), and transwell assay ( D ) showed that the enhanced cell proliferation and migration of LUAD cells induced by knocking down RBMS3-AS3 were partly restored by ZEB1 overexpression. E The xenograft tumor assay with A549 cells was carried out to determine the effect of ZEB1 on tumorigenesis induced by stably knocking down RBMS3-AS3 in vivo. F Tumor volume was measured and recorded every 4 days and the curve was plotted. G The tumors were weighed. H , I The reduced lipid ROS level caused by silencing RBMS3-AS3 was rescued by overexpressing ZEB1 in A549 and PC9 cells with the treatment of RSL3. J – L ZEB1 overexpression partially restored the decreased intracellular ferroptosis level induced by RBMS3-AS3 knockdown via detecting the GSH/GSSG ratio ( J ), Fe 2+ level ( K ), and MDA level ( L ). Data were representative images or were expressed as the mean ± standard deviation. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: NPJ Precision Oncology

Article Title: M6A-ALKBH5-dependent RBMS3-AS3 down-regulation suppresses ferroptosis to promote lung adenocarcinoma progression through HNRNPDL/ZEB1/GPX4 axis

doi: 10.1038/s41698-025-01141-y

Figure Lengend Snippet: A – D The rescue CCK-8 assay ( A ), colony formation assay ( B ), EdU staining assay ( C ), and transwell assay ( D ) showed that the enhanced cell proliferation and migration of LUAD cells induced by knocking down RBMS3-AS3 were partly restored by ZEB1 overexpression. E The xenograft tumor assay with A549 cells was carried out to determine the effect of ZEB1 on tumorigenesis induced by stably knocking down RBMS3-AS3 in vivo. F Tumor volume was measured and recorded every 4 days and the curve was plotted. G The tumors were weighed. H , I The reduced lipid ROS level caused by silencing RBMS3-AS3 was rescued by overexpressing ZEB1 in A549 and PC9 cells with the treatment of RSL3. J – L ZEB1 overexpression partially restored the decreased intracellular ferroptosis level induced by RBMS3-AS3 knockdown via detecting the GSH/GSSG ratio ( J ), Fe 2+ level ( K ), and MDA level ( L ). Data were representative images or were expressed as the mean ± standard deviation. * p < 0.05, ** p < 0.01, *** p < 0.001.

Techniques: CCK-8 Assay, Colony Assay, Staining, Transwell Assay, Migration, Over Expression, Stable Transfection, In Vivo, Knockdown, Standard Deviation

A The correlation coefficient of ZEB1 and GPX4 in LUAD tumors from the TCGA cohort. B , C The expression of GPX4 was examined by RT-qPCR ( B ) and WB ( C ) in A549 and PC9 cells subjected to the knockdown or overexpression of ZEB1. D The ZEB1 binding sites in the GPX4 promoter were predicted by JASPAR. E , F The products of the ChIP assay in LUAD cells were checked by agarose gel electrophoresis ( E ) and RT-qPCR ( F ). G , H Knocking down RBMS3-AS3 in A549 cells led to a reduction in GPX4 enrichment by ZEB1. I , J The effect of RBMS3-AS3 on GPX4 expression was detected by RT-qPCR ( I ) and WB ( J ) in LUAD cells. K , L ZEB1 overexpression restored the increased GPX4 expression induced by silencing RBMS3-AS3. M The schematic of the GPX4 promoter with luciferase reporter vectors. N The Dual-luciferase assay was conducted to assess the relative luciferase activity of reporter plasmids containing the GPX4 promoter and its mutant type in ZEB1 overexpression LUAD cells. Data were representative images or were expressed as the mean ± standard deviation. ns: no significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: NPJ Precision Oncology

Article Title: M6A-ALKBH5-dependent RBMS3-AS3 down-regulation suppresses ferroptosis to promote lung adenocarcinoma progression through HNRNPDL/ZEB1/GPX4 axis

doi: 10.1038/s41698-025-01141-y

Figure Lengend Snippet: A The correlation coefficient of ZEB1 and GPX4 in LUAD tumors from the TCGA cohort. B , C The expression of GPX4 was examined by RT-qPCR ( B ) and WB ( C ) in A549 and PC9 cells subjected to the knockdown or overexpression of ZEB1. D The ZEB1 binding sites in the GPX4 promoter were predicted by JASPAR. E , F The products of the ChIP assay in LUAD cells were checked by agarose gel electrophoresis ( E ) and RT-qPCR ( F ). G , H Knocking down RBMS3-AS3 in A549 cells led to a reduction in GPX4 enrichment by ZEB1. I , J The effect of RBMS3-AS3 on GPX4 expression was detected by RT-qPCR ( I ) and WB ( J ) in LUAD cells. K , L ZEB1 overexpression restored the increased GPX4 expression induced by silencing RBMS3-AS3. M The schematic of the GPX4 promoter with luciferase reporter vectors. N The Dual-luciferase assay was conducted to assess the relative luciferase activity of reporter plasmids containing the GPX4 promoter and its mutant type in ZEB1 overexpression LUAD cells. Data were representative images or were expressed as the mean ± standard deviation. ns: no significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Techniques: Expressing, Quantitative RT-PCR, Knockdown, Over Expression, Binding Assay, Agarose Gel Electrophoresis, Luciferase, Activity Assay, Mutagenesis, Standard Deviation

A , B The subcellular localization of RBMS3-AS3 was determined by FISH assay ( A ) and nucleocytoplasmic separation assay ( B ). C The RNA pull-down assay was conducted in LUAD cells with the biotinylated-RBMS3-AS3 and antisense probe, and subsequently subjected to SDS-PAGE with silver staining. D The results of mass spectrometry identified 20 RBPs that were only pulled down by RBMS3-AS3 probes in LUAD cells. E The binding of RBMS3-AS3 and HNRNPDL was validated by WB using RNA pull-down samples and RIP assay. F , G The nuclear and cytoplasmic proteins ( F ) and RNAs ( G ) were extracted to examine the subcellular localization of HNRNPDL by WB and RT-qPCR. H The co-localization of RBMS3-AS3 and HNRNPDL in A549 cells subjected to RBMS3-AS3 knockdown or overexpression. I In silico protein-RNA molecular docking simulations. J Illustration of full-length Flag-labeled HNRNPDL and other mutants. K RIP assays quantified RBMS3-AS3 enrichment bound to Flag-tagged HNRNPDL (full-length and truncated variants). L Secondary structure of RBMS3-AS3 predicted by RNAfold Website. M The RNA pull-down experiments using three truncated fragments of RBMS3-AS3 were performed. N The half-life of ZEB1 mRNA was measured by RT-qPCR in HNRNPDL knockdown or overexpression LUAD cells and this effect could be rescued by overexpressing or knocking down RBMS3-AS3, respectively. O The schematic of luciferase reporter vectors containing the ZEB1 mRNA 3′-UTR or its mutant variant. P The Dual-luciferase assay was conducted to assess the relative luciferase activity of reporter plasmids containing ZEB1 mRNA 3′-UTR or its mutant type in HNRNPDL knockdown LUAD cells. Data were representative images or were expressed as the mean ± standard deviation. ns: no significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: NPJ Precision Oncology

Article Title: M6A-ALKBH5-dependent RBMS3-AS3 down-regulation suppresses ferroptosis to promote lung adenocarcinoma progression through HNRNPDL/ZEB1/GPX4 axis

doi: 10.1038/s41698-025-01141-y

Figure Lengend Snippet: A , B The subcellular localization of RBMS3-AS3 was determined by FISH assay ( A ) and nucleocytoplasmic separation assay ( B ). C The RNA pull-down assay was conducted in LUAD cells with the biotinylated-RBMS3-AS3 and antisense probe, and subsequently subjected to SDS-PAGE with silver staining. D The results of mass spectrometry identified 20 RBPs that were only pulled down by RBMS3-AS3 probes in LUAD cells. E The binding of RBMS3-AS3 and HNRNPDL was validated by WB using RNA pull-down samples and RIP assay. F , G The nuclear and cytoplasmic proteins ( F ) and RNAs ( G ) were extracted to examine the subcellular localization of HNRNPDL by WB and RT-qPCR. H The co-localization of RBMS3-AS3 and HNRNPDL in A549 cells subjected to RBMS3-AS3 knockdown or overexpression. I In silico protein-RNA molecular docking simulations. J Illustration of full-length Flag-labeled HNRNPDL and other mutants. K RIP assays quantified RBMS3-AS3 enrichment bound to Flag-tagged HNRNPDL (full-length and truncated variants). L Secondary structure of RBMS3-AS3 predicted by RNAfold Website. M The RNA pull-down experiments using three truncated fragments of RBMS3-AS3 were performed. N The half-life of ZEB1 mRNA was measured by RT-qPCR in HNRNPDL knockdown or overexpression LUAD cells and this effect could be rescued by overexpressing or knocking down RBMS3-AS3, respectively. O The schematic of luciferase reporter vectors containing the ZEB1 mRNA 3′-UTR or its mutant variant. P The Dual-luciferase assay was conducted to assess the relative luciferase activity of reporter plasmids containing ZEB1 mRNA 3′-UTR or its mutant type in HNRNPDL knockdown LUAD cells. Data were representative images or were expressed as the mean ± standard deviation. ns: no significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Techniques: Pull Down Assay, SDS Page, Silver Staining, Mass Spectrometry, Binding Assay, Quantitative RT-PCR, Knockdown, Over Expression, In Silico, Labeling, Luciferase, Mutagenesis, Variant Assay, Activity Assay, Standard Deviation

A – F CCK-8 ( A ), colony formation ( B , C), EdU staining ( D , F ), and transwell assay ( E , F ) were carried out to assess the cell functions of ZEB1 overexpression in LUAD cells with HNRNPDL1 silencing. G , H HNRNPDL knockdown in LUAD cells rescued the ZEB1 overexpression-induced decrease in GPX4 expression, as measured by RT-qPCR and WB. I The GPX4 protein levels shown in Fig.8H were quantified using ImageJ software. J – N The effect of silencing HNRNPDL on the enhanced ferroptosis level caused by overexpressing ZEB1 in LUAD cells treated with RSL3 was examined by detecting the lipid ROS level ( J , K ), Fe 2+ level ( L ), GSH/GSSG ratio ( M ), and MDA level ( N ). Data were representative images or were expressed as the mean ± standard deviation. ns: no significant, * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: NPJ Precision Oncology

Article Title: M6A-ALKBH5-dependent RBMS3-AS3 down-regulation suppresses ferroptosis to promote lung adenocarcinoma progression through HNRNPDL/ZEB1/GPX4 axis

doi: 10.1038/s41698-025-01141-y

Figure Lengend Snippet: A – F CCK-8 ( A ), colony formation ( B , C), EdU staining ( D , F ), and transwell assay ( E , F ) were carried out to assess the cell functions of ZEB1 overexpression in LUAD cells with HNRNPDL1 silencing. G , H HNRNPDL knockdown in LUAD cells rescued the ZEB1 overexpression-induced decrease in GPX4 expression, as measured by RT-qPCR and WB. I The GPX4 protein levels shown in Fig.8H were quantified using ImageJ software. J – N The effect of silencing HNRNPDL on the enhanced ferroptosis level caused by overexpressing ZEB1 in LUAD cells treated with RSL3 was examined by detecting the lipid ROS level ( J , K ), Fe 2+ level ( L ), GSH/GSSG ratio ( M ), and MDA level ( N ). Data were representative images or were expressed as the mean ± standard deviation. ns: no significant, * p < 0.05, ** p < 0.01, *** p < 0.001.

Techniques: CCK-8 Assay, Staining, Transwell Assay, Over Expression, Knockdown, Expressing, Quantitative RT-PCR, Software, Standard Deviation

ALKBH5 downregulated RBMS3-AS3 expression in LUAD by the m 6 A modification. The decreased expression of RBMS3-AS3 promoted tumor progression and suppressed intracellular ferroptosis levels in LUAD via the HNRNPDL/ZEB1/GPX4 axis.

Journal: NPJ Precision Oncology

Article Title: M6A-ALKBH5-dependent RBMS3-AS3 down-regulation suppresses ferroptosis to promote lung adenocarcinoma progression through HNRNPDL/ZEB1/GPX4 axis

doi: 10.1038/s41698-025-01141-y

Figure Lengend Snippet: ALKBH5 downregulated RBMS3-AS3 expression in LUAD by the m 6 A modification. The decreased expression of RBMS3-AS3 promoted tumor progression and suppressed intracellular ferroptosis levels in LUAD via the HNRNPDL/ZEB1/GPX4 axis.

Techniques: Expressing, Modification

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